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analytical spectroscopy by raymond p. w. scott d.sc., f.r.s.c., c.chem., c.sci. f.a.i.c, f.c.s. essential information for the analytical chemist tools wavenumber calculator cm -1 = m = ev = hz meteormetrics limited specialising in custom-designed, precision scientific instruments, built, programmed and calibrated to the most exacting standards. the range includes precision dataloging barographs, with built-in statistical analysis, barographic transient event recorders and computer-interfaced detectors and sensors for environmental monitoring & process control. drkfs.net a site dedicated to scientific techniques, experimental methods, & investigative tools for the inventor, researcher and laboratory pioneer. articles on glassblowing, electronics, metalcasting, magnetic measurements with new material added continually. check it out! www.drkfs.net analytical spectroscopy welcome to analytical spectroscopy online! the author and the publ;isher of this work hopes that it will provide essential information to practising analytical chemists, to students and to technicians involved in the far-ranging application of spectroscopic techniques. the books published here are, therefore, not primarily intended for the expert spectroscopist but, on the contrary for the analyst who may be relatively inexperienced in spectroscopic techniques, the theory behind them, their areas of application and the type of instrumentation that is necessary for their successful use. the material is copyright and can be downloaded and copied by individuals for their own use but it must not be reproduced in hard copy or in electronic form for any commercial purpose, nor presented without due acknowledgement of the author. the series includes eight individual monographs. 1. uv and visible spectroscopy 2. fluorescence spectroscopy 3. infrared spectroscopy 4. raman spectroscopy 5. atomic spectroscopy 6. electron spin ~spectroscopy (yet to be published) 7.nuclear magnet resonance spectroscopy 8.mass spectroscopy the treatment of spectroscopy proceeds logically, starting in book 1 with a discussion of electromagnetic waves, progressing to the principles involved in the absorption and generation of electromagnetic radiation and then describes, perhaps, the simplest form of the technique, i.e . uv and visible spectroscopy. in the second book general luminescence is discussed dealing specifically with that particular form of luminescence , fluorescence and its use in analytical chemistry. in the third book, the arguments and discussions presented in sections 1 and 2 are extended to electromagnetic radiation of longer wavelengths viz. infrared and raman spectroscopy . the subject continues to be developed logically through atomic spectroscopy and other spectroscopic techniques finally dealing with mass spectroscopy actually, mass spectrometry is not truly a spectroscopic technique as it does not involve electromagnetic radiation but, it is important in structure elucidation, so for convenience, it is included with other true spectroscopic methods. at all stages suitable instrumentation is described and special methods of operation are discussed back index home about the author links uv-vis 1-1 introduction 1-2 the spectrum 1-3 absorption of light i 1-4 absorption of light ii 1-5 practical aspects of uv/vis spectroscopy 1-6 basic uv/vis spectrometer 1-7 uv/vis light sources 1-8 lens and window materials 1-9 sample cells 1-10 references fluor 2-1 forms of light emission 2-2 mechanism of fluorescence 2-3 phosphore- scence 2-4 light scattering 2-5 rayleigh-tyndale scattering 2-6 raman scattering 2-7 derivatization 2-8 constituents of a fluorescence spectometer 2-9 excitation light sources 2-10 light sensors 2-11 the fluorescence spectrometer in practice 2-12 instrumentation 2-13 experimental fluorescence spectroscopy 2-14 examples of special applications 2-15 the multiwave fluorescence detector 2-16 references ir 3-1 electromagnetic spectrum in chemistry 3-2 introduction to ir 3-3 molecular vibrations 3-4 normal modes 3-5 sample effects 3-6 ir instruments 3-7 simple grating ir spectrometer 3-8 fourier transform ir spectrometer. 3-9 sampling procedures 3-10 reflectance methods 3-11 diffuse reflectance ir ft spectrometry 3-12 photo acoustic spectroscopy 3-13 a commercial ir spectrometer 3-14 application of ft-ir to a forensic problem 3-15 the basic interferometer unit 3-16 references raman 4-1 raman scattering 4-2 raman spectroscopy instrumentation 4-3 sensors and detection 4-4 sample handling 4-5 ftir raman spectrometer system 4-6 synchrotron sources for raman spectrometry 4-7 polarization effects in raman spectroscopy 4-8 references atomic 5-1 atomic spectroscopy 5-2 atomic emission spectroscopy 5-3 atomic emission spectrometer 5-4 hp plasma atomic emission spectrometer 5-5 shimadzu icpe-9000 spectrometer 5-6 atomic absorption spectroscopy 5-7 flame atomic absorption spectrometer 5-8 electrothermal atomization 5-9 graphite furnace 5-10 the l’vov platform 5-11 atomic absorption spectra 5-12 zeeman effect in reducing interference 5-13 atomic spectroscopy gc tandem system 5-14 references nmr 7-1 nuclear magnetic resonance spectroscopy 7-2 nmr spectrometer 7-3 a high resolution nmr machine 7-4 sample solvents 7-5 chemical shifts 7-6 references ms i 8-1 introduction to mass spectrometry 8-2 ionization methods in ms 8-3 electron impact ionization 8-4 chemical ionization 8-6 secondary ion mass spectrometry (sims) 8-7 fast atom bombardment (fab) 8-8 plasma desorption ms 8-9 inductively coupled plasma ionization source 8-10 laser desorption ionization 8-12 field desorption ionization 8-13 ionization systems for liquid samples ms ii 8-14 direct inlet interfaces 8-15 thermospray interface 8-16 electrospray liquid sampling system 8-17 applications of electrospray ionization 8-18 atmospheric ionization interface(api) 8-19 inductively coupled plasma ionization sources 8-20 particle beam interface 8-21 permeable membrane interface 8-22 sector ms 8-23 quadrupole ms 8-25 time of flight ms 8-26 references next about the author raymond peter william scott was born on june 20 1924 in erith, kent, uk. he studied at the university of london, obtaining his b.sc. degree in 1946 and his d.sc. degree in 1960. after spending more than a decade at benzole producers, ltd. where he became head of the physical chemistry laboratory, he moved to unilever research laboratories as manager of their physical chemistry department. in 1969 he became director of physical chemistry at hoffmann-la roche, nutley, nj, u.s.a. and subsequently accepted the position of director of the applied research department at the perkin-elmer corporation, norwalk, ct, u.s.a. in 1986 he became an independent consultant and was appointed visiting professor at georgetown university, washington, dc, u.s.a. and at berkbeck college of the university of london; in 1986 he retired but continues to write technical books dealing with various aspects of physical chemistry and physical chemical techniques. dr. scott has authored or co-authored over 200 peer reviewed scientific papers and authored, co-authored or edited over thirty books on various aspects of physical and analytical chemistry. dr. scott was a founding member of the british chromatography society and received the american chemical society award in chromatography (1977), the m. s. tswett chromatography medal (1978), the tswett chromatography medal u.s.s.r., (1979), the a. j. p. martin chromatography award (1982) and the royal society of chemistry award in analysis and instrumentation (1988). dr. scott’s activities in gas chromatography started at the inception of the technique, inventing the heat of combustion detector (the precursor of the flame ionization detector), pioneered work on high sensitivity detectors, high efficiency columns and presented fundamental treatments of the relationship between the theory and practice of the technique. he established the viability of the moving bed conti

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